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本文报道几年来分离筛选的一些固氮菌株在相应的水稻品种上的接种效应。在设置试验中改变了一般采用的缺氮试验方法,供给水稻生长以适当的氮素营养,使其根分泌物增多,借以作为根系固氮微生物生长和固氮的营养料。盆栽试验结果初步表明:1.经灌浆期测定,接种于水稻相应品种各个生育期的土壤中,接种菌都有明显的根际效应,都能群集于水稻根系的表面,有些甚至进入根内。扬花期及孕穗期接种的群集现象最显著,其它时期接种的较差;而固氮酶活性则分蘖期最高,其次为扬花期,苗期最低,但土壤中检测不到接种菌及固氮酶活性。黄熟期测定的菌数和固氮酶活性都比灌浆期测定的低,而且土壤中也可检测到少量固氮菌和固氮酶活性。2.三个水稻品种接种相应的分离菌,其根际效应也很明显,同样能大量群集于根表。接种的根表菌数比不接种的高15～30倍以上。3.两个有效菌株接种不同水稻品种对产量影响的盆栽试验表明,菌株243(?)适应性较广,在接种的五个品种中四个表现增产,其幅度为6～22.9％,而一菌株则不明显。
This article reports the sequestration effects of some nitrogen-fixing strains isolated and screened over the past few years on the corresponding rice varieties. In setting experiments, the commonly used method of nitrogen deficiency test was changed to supply rice with proper nitrogen nutrition to increase its root exudates so as to serve as nutrition material for nitrogen fixation and nitrogen fixation of roots. The preliminary results of pot experiment showed that: 1. The inoculation period was measured in the filling stage and inoculated into the soil of each growth stage of the corresponding rice cultivars. The inoculation bacteria all had obvious rhizosphere effect and clustered on the surface of rice root system, and some even entered the root. Inoculation at flowering stage and booting stage was the most significant, while in other stages the inoculation was poor. The activity of nitrogenase was the highest at tillering stage, followed by flowering stage and the lowest at seedling stage. However, inoculation bacteria and nitrogenase activity were not detected in soil. The number of bacteria and nitrogenase activity in yellow ripe stage were lower than those in the filling stage, and a small amount of nitrogen-fixing bacteria and nitrogenase activity could also be detected in the soil. 2. Three rice varieties inoculated with the corresponding isolates, the rhizosphere effect is also very obvious, but also a large number of clusters in the root table. The number of inoculated rhizome bacteria is 15-30 times higher than that of non-vaccinated ones. Pot experiments of two effective strains inoculated with different rice varieties on the yield showed that the strain 243 (?) Had a wide adaptability, and four of the five varieties inoculated showed an increase in yield of 6-22.9% Strain is not obvious.