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目的:通过检测四逆散不同配伍中有效成分柴胡皂苷a在大鼠体内的药代动力学参数,发现药物之间的协同或拮抗作用,探讨四逆散配伍的合理性。方法:通过灌胃分别给予大鼠四逆散全方汤剂及7组拆方配伍汤剂,使用液相色谱仪检测柴胡皂苷a的血药浓度,利用计算机软件计算其药代动力学参数。结果:白芍与柴胡配伍明显提高了柴胡皂苷a在大鼠体内的最大浓度含量;炙甘草与柴胡配伍不仅提高了柴胡皂苷a在大鼠体内的最大浓度含量,还延长了柴胡皂苷a在大鼠体内的半衰期;枳实本身与柴胡配伍对大鼠体内柴胡皂苷a的药代动力学参数影响不明显,但多药合用则对柴胡皂苷a的半衰期有明显延长作用。综合比较各项参数,对四逆散中柴胡有效成分柴胡皂苷a的吸收率及血药浓度维持最为有益的配伍为四逆散全方。结论:四逆散全方结构严谨配伍精妙,白芍和炙甘草均对柴胡的功效具有积极作用,可促进其有效成分的吸收,并与枳实产生协同作用,共同维持柴胡中有效成分柴胡皂苷a的血药浓度,其组方配伍具有很高的科学性和合理性。
Objective: To detect the pharmacokinetic parameters of Saikosaponin-a in different compatibility of Sini powder in rats, to find out the synergistic or antagonistic effect between drugs and to explore the rationality of compatibility of Siniosan. Methods: The rats were given intragastric administration of Sini Powder Decoction and seven groups decoction compatibility decoction, the use of liquid chromatography to detect saikosaponin a blood concentration, the use of computer software to calculate the pharmacokinetic parameters . Results: Compatibility of Radix Paeoniae Radix and Radix Bupleuri increased the maximum concentration of Saikosaponin a in rats. The compatibility of Radix Bupleuri and Radix Bupleuri increased the maximum concentration of Saikosaponin a in rats, The half-life of the saikosaponin a in vivo. The pharmacokinetics parameters of the saikosaponin a were not significantly affected by the combination of the citrus aurantium itself and the Bupleurum. However, the half-life of saikosaponin a was significantly prolonged effect. Comprehensive comparison of various parameters, the Sini powder Bupleurum active ingredients Saikosaponin a absorption rate and plasma concentration to maintain the most beneficial compatibility for the Sini powder all. CONCLUSIONS: Sini Powder has the rigorous and rigorous compatibility of all-round structure. Both peony root and Zhigancao have a positive effect on the efficacy of Bupleurum, which can promote the absorption of its active ingredients and synergize with Citrus aurantium to maintain the active ingredients in Bupleurum Saikosaponin a blood concentration, the compatibility of its prescription is highly scientific and reasonable.