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目的 研究锌对肝脏缺血再灌注损伤 (HIRI)保护作用的分子机制。方法 采用RT- PCR技术 ,检测分析金属硫蛋白 - 1 (MT- 1 )基因在 HIRI大鼠肝组织中的表达和锌对其表达的调节。结果 各组大鼠肝组织均有MT- 1基因表达 ;缺血 30 min、再灌 90 min大鼠肝组织中 MT- 1m RNA较对照组显著降低 (P<0 .0 1 ) ;灌胃补锌后 ,HIRI大鼠肝 MT- 1 m RNA转录水平较对照组明显增高 (P<0 .0 1 ) ,单纯补锌亦可上调其组织中 MT- 1基因表达 (P<0 .0 1 )。结论 在锌对HIRI产生保护作用的分子机制中 ,调节其肝组织中 MT- 1转录水平的表达可能是一条重要途径。
Objective To study the molecular mechanism of zinc on hepatic ischemia-reperfusion injury (HIRI). Methods RT-PCR was used to detect the expression of metallothionein-1 (MT-1) gene in liver tissue of HIRI rats and the regulation of zinc expression. Results The expression of MT-1 gene in liver tissue of rats in each group was significantly lower than that in control group (P <0.01). The levels of MT-1 mRNA in liver tissue of rats after 30 min of ischemia and 90 min of reperfusion were significantly decreased Zinc, HIRI rat liver MT-1 m RNA transcription levels were significantly higher than the control group (P <0.01), zinc alone can also increase its tissue MT-1 gene expression (P <0.01) . Conclusion In the molecular mechanism of the protective effect of zinc on HIRI, regulating the expression of MT-1 in liver may be an important pathway.