目的 :构建噬菌体抗体库并筛选抗黑素瘤细胞的人抗体。方法 :从 1例长期存活的黑素瘤患者的外周血中分离单个核细胞 (PBMC) ,提取总RNA ,并逆转录为cDNA。用PCR扩增多样性κ链基因和重链Fd段基因 ,分别构建κ链库和重链库 ,组合为Fab段噬菌体抗体库 ,用两种黑素瘤细胞系对抗体库进行筛选和鉴定。结果 :所构建的噬菌体抗体库的库容量达 1.2× 10 8,用两种黑素瘤细胞系进行亲和吸附筛选 ,获得针对其中 1种黑素瘤细胞系的特异性抗体。结论 :采用噬菌体抗体库技术 ,成功地从 1例长期存活的黑素瘤患者体内克隆到特异性抗黑素瘤细胞的抗体 ,为进一步的研究和应用奠定了基础 Objective: To construct a phage antibody library and screen human antibodies against melanoma cells. Methods: Mononuclear cells (PBMCs) were isolated from the peripheral blood of 1 long-term melanoma patients, total RNA was extracted and reverse transcribed into cDNA. The PCR products were used to amplify the genes encoding the κ chain and the Fd gene. The κ chain library and heavy chain library were constructed respectively, and the Fab fragment phage antibody library was constructed. The antibody library was screened and identified by using two melanoma cell lines. Results: The constructed phage antibody library had a capacity of 1.2 × 10 8, and was screened by two kinds of melanoma cell lines to get the specific antibodies against one of the melanoma cell lines. CONCLUSIONS: The phage antibody library technique was used to successfully clone specific anti-melanoma cells from one patient with long-term survival melanoma and lay the foundation for further research and application