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目的探讨正畸牙齿移动与肿瘤坏死因子α(TNF-α)表达的关系。方法 2008年9月至2009年1月于大连医科大学基础实验室,选取8周龄雄性SD大鼠18只,体重180～220g。以大鼠上颌左侧牙弓为实验侧,右侧为对照侧。实验侧以大鼠上颌切牙为支抗牙,0.392N(40gf)力拉上颌第一磨牙向近中;对照侧不做任何处理。正畸加力第1、3、5、7、14、21天分别处死3只大鼠,取上颌双侧磨牙及周围组织制备切片,进行苏木素-伊红(HE)染色和抗酒石酸酸性磷酸酶(TRAP)染色,并采用免疫组化SABC法检测TNF-α变化(以第5天实验侧切片为阴性对照)。结果正畸牙齿移动的骨改建过程中TNF-α水平随时间的增加呈先上升后下降的趋势。正常大鼠牙周组织中偶见TNF-α的表达,阴性对照未见TNF-α的表达。实验侧TNF-α的表达在加力第5天达到峰值,第21天接近对照侧的水平。结论 OC的形成分化和骨吸收的发生密切相关,是引发骨吸收的主要细胞;TNF-α是调控OC的形成和分化的重要因子,在正畸牙齿移动中发挥重要作用。
Objective To investigate the relationship between orthodontic tooth movement and tumor necrosis factor α (TNF-α) expression. METHODS: From September 2008 to January 2009, 18 male SD rats, 8 weeks old, weighing 180-220 g were enrolled in the basic laboratory of Dalian Medical University. The left maxillary arch was the experimental side and the right was the control side. The experimental side of the rat maxillary incisors as the supporting teeth, 0.392N (40gf) pull the maxillary first molar in the near middle; the control side without any treatment. Three rats were killed on the 1st, 3rd, 5th, 7th, 14th and 21st day after orthodontic treatment. The bilateral maxillary molars and surrounding tissues were prepared and stained with hematoxylin and eosin (HE) and anti-tartrate acid phosphatase (TRAP) staining, and immunohistochemical SABC assay TNF-α changes (the experimental side of the fifth day as a negative control). Results In orthodontic tooth movement, the level of TNF-α increased first and then decreased with the increase of time. The expression of TNF-α was found occasionally in normal periodontal tissues and no TNF-α was found in the negative control. Experimental side of the expression of TNF-α peaked on the 5th day after the force, on the 21st day close to the level of the control side. Conclusion The formation and differentiation of OC is closely related to the occurrence of bone resorption and is the main cell that induces bone resorption. TNF-α is an important factor in regulating the formation and differentiation of OC, and plays an important role in orthodontic tooth movement.