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目的 探讨纳米磁性微粒的合成制备方法及其在酶联免疫吸附试验中的初步应用。方法 采用共沉淀法制备了纳米Fe3O4磁性微粒 ,并以此为核心进行无皂乳液聚合 ,聚合后的磁性微粒表面化学接枝蛋白质 ,并探讨其在酶联免疫吸附试验中的初步应用。结果 共沉淀法合成的Fe3O4平均粒径 12 .2± 4.4nm( 6～ 2 4nm) ,聚合后的复合磁性微粒平均粒径 48.5± 10 .0nm( 2 8～ 70nm) ,表面带有羧基活性基团 ,利用羧基与蛋白质氨基形成的共价键 ,接枝辣根过氧化物酶 羊抗人IgG或人工表达的解脲脲原体膜抗原MBA于磁性微粒表面。键合标记物的复合磁性微粒具有较高的辣根过氧化物酶活性 ,并可用于酶联免疫吸附试验 ,检测血清中抗解脲脲原体抗体。结论 纳米级磁性微粒具有表面标记蛋白质的特异性 ,还具有纳米微粒表面积大的敏感性和磁性分离的方便性 ,有很大的医学应用前途。
Objective To study the synthesis and preparation of nano-magnetic particles and its preliminary application in enzyme-linked immunosorbent assay. Methods Nanometer Fe3O4 magnetic particles were prepared by co-precipitation method. Soap-free emulsion polymerization was used as the core. The chemically grafted protein on the surface of the magnetic particles after polymerization was investigated. The preliminary application of the method in enzyme-linked immunosorbent assay (ELISA) was also discussed. Results The average particle diameter of Fe3O4 synthesized by coprecipitation method was 12.2 ± 4.4nm (6 ~ 24nm). The average size of the composite magnetic particles after polymerization was 48.5 ± 10.0nm (28 ~ 70nm) Mission, the use of carboxyl and protein amino groups form a covalent bond, grafted horseradish peroxidase goat anti-human IgG or artificially expressed UU membrane antigen MBA in the magnetic particle surface. The labeled magnetic particles of composite magnetic particles have higher horseradish peroxidase activity, and can be used for enzyme-linked immunosorbent assay to detect anti-Ureaplasma urealyticum antibody in serum. Conclusion Nanoscale magnetic particles have the specificity of surface-labeled proteins. They also have the advantages of large surface area of nanoparticles and convenience of magnetic separation, which have great medical application prospects.