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目的通过检测IL-6与骨密度及骨小梁形成之间的关系,进一步验证JAK2/STAT3信号通路在骨质疏松中的作用机制。方法选取100例骨质疏松患者(OP组)和100例非骨质疏松者(NOP组),通过骨密度分析仪测定骨密度,静脉取外周血通过酶联免疫分析方法和全自动生化分析仪检测血清IL-6及碱性磷酸酶(Alkaline Phosphatase,ALP)水平。通过卵巢切除术构建大鼠骨质疏松模型,随机分为未阻断组、JAK/STAT阻断组、假手术组,分离静脉血及处死后分离股骨及胸椎骨通过ELISA及组织HE染色检测IL-6及骨小梁的情况。结果 OP组血清IL-6水平[(193.45±35.78)pg/ml]显著高于NOP组[(114.23±41.12)pg/ml](t=12.183,P<0.001)。大鼠模型JAK/STAT未阻断组骨密度[(0.499±0.21)g/cm2]与ALP水平[(66.43±9.18)U/L]较假手术组均明显降低(F=0.207,22.689;P<0.001),说明造模成功。而大鼠模型JAK/STAT阻断组的骨密度[(0.707±0.19)g/cm2]与ALP水平[(89.67±8.98)U/L]明显高于未阻断组(F=0.215,26.464;P<0.001);HE染色显示JAK/STAT阻断组骨小梁的密度及质量明显高于未阻断组。结论 JAK2/STAT3信号途径影响骨小梁形成,并以此下调骨密度从而在骨质疏松症的发生及发展过程中发挥着重要的作用。
Objective To further verify the mechanism of JAK2 / STAT3 signaling pathway in osteoporosis by detecting the relationship between IL-6 and bone mineral density and trabecular formation. Methods 100 osteoporosis patients (OP group) and 100 non-osteoporosis patients (NOP group) were selected. BMD was measured by bone mineral density analyzer, Serum levels of IL-6 and Alkaline Phosphatase (ALP) were detected. The model of osteoporosis was established by ovariectomy. The rats were randomly divided into non-blocking group, JAK / STAT blocking group, sham operation group, venous blood separation and femur and thoracic bone after sacrifice. ELISA and HE staining were used to detect IL -6 and trabecular bone. Results The serum level of IL-6 in OP group [(193.45 ± 35.78) pg / ml] was significantly higher than that in NOP group [(114.23 ± 41.12) pg / ml] (t = 12.183, P <0.001). Compared with sham operation group, the bone mineral density ([(0.499 ± 0.21) g / cm2] and ALP level [(66.43 ± 9.18) U / L] in JAK / <0.001), indicating successful modeling. However, the BMD of JAK / STAT block group was significantly higher than that of non-block group (F = 0.215,26.464; [0.707 ± 0.19] g / cm2] and ALP level [(89.67 ± 8.98) U / L] P <0.001). The HE staining showed that the density and the quality of trabecular bone in the JAK / STAT block group were significantly higher than those in the non-block group. Conclusions The JAK2 / STAT3 signaling pathway affects the formation of trabecular bone and thus down-regulates bone mineral density and thus plays an important role in the development of osteoporosis.