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目的研究副肿瘤综合征(PNS)自身抗体(抗Hu抗体)对培养神经细胞的直接影响。方法抗Hu抗体阳性PNS患者血清IgG、对照组血清IgG添加到由胎鼠脑分离出的培养神经细胞,观察有无细胞的凋亡。对照组血清IgG为：(1)AB型健康人标准血清；(2)无神经损害症状的抗Hu抗体阴性痛患者血清；(3)重组Hu蛋白吸附后的抗Hu抗体阳性患者血清。结果添加抗Hu抗体阳性PNS患者血清IgG的培养神经细胞互相融合,由粗而长的突起相互连接,而对照组未看到上述变化。各组均未发现凋亡的神经细胞。结论抗Hu抗体未能引起神经细胞的凋亡或死亡,推测是诱导了接触因子的表达促进了细胞的分化。
Objective To study the direct effects of autoantibodies against PNS (anti-Hu antibody) on cultured neurons. Methods Serum IgG of anti-Hu antibody-positive PNS patients and serum IgG of control group were added to the cultured nerve cells isolated from fetal rat brain to observe the presence or absence of apoptosis. The serum IgG of the control group was: (1) AB type healthy human serum; (2) serum of anti-Hu antibody negative pain without neurological damage; (3) serum of anti-Hu antibody positive patients after adsorption of recombinant Hu protein. Results Cultured neurons of serum IgG with anti-Hu antibody-positive PNS were fused with each other and were connected with each other by thick and long protuberances. The above changes were not observed in the control group. Apoptotic nerve cells were not found in all groups. Conclusion Anti-Hu antibody failed to induce apoptosis or death of nerve cells, suggesting that induction of contact factor expression promotes cell differentiation.