:本文介绍一种快速敏感的原位杂交方法。用光敏生物素标记管蛋白cDNA探针,经DNase I酶切成组织渗透性强适合原位杂交的短片段,作快速的原位杂交,再用胶体金标链亲和素系统检测杂交结果。整个过程操作方便,迅速,能保存较多的杂交信号,杂交结果清晰。 : This article presents a quick and sensitive in situ hybridization method. Using photobiotin labeled tube protein cDNA probe, digested by DNase I into tissue-penetrating strong for in situ hybridization of short fragments for rapid in situ hybridization, and then colloidal gold labeled streptavidin system hybridization results. The whole process is easy to operate, rapid, can save more hybridization signals, hybridization results clear.