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【目的】测定6株戴氏霉(Taifanglania)的生长温度特性及其对秸秆的降解效果。【方法】通过定时测定不同培养温度下的菌落直径绘制6株戴氏霉菌株的生长曲线;采用苯胺蓝法、愈创木酚法和木质素磺酸钙降解试验测定其木质素降解能力;用羧甲基纤维素钠水解圈测定法和胞外酶活测定法判定其对纤维素的降解能力;以失重法和范氏洗涤剂法检测其对水稻秸秆的降解效果。【结果】所试的耐热戴氏霉菌株均能耐受50°C的高温,并能产生纤维素酶,但不同菌株产生的木质素降解酶有所差异;均具有降解秸秆的能力,其中合川戴氏霉(T.hechuanensis)H08.1菌株降解能力最强,其次是灰戴氏霉(T.cinerea)H57.1菌株,其秸秆降解率分别为50.2%和42.2%。【结论】合川戴氏霉H08.1菌株和灰戴氏霉H57.1菌株在秸秆的降解利用上具有潜在开发价值。
【Objective】 The objective of this study was to determine the growth temperature characteristics of 6 strains of Taifanglania and their effects on the degradation of straw. 【Method】 The growth curve of 6 strains of Daphnia magna was drawn by regularly measuring the colony diameters at different culturing temperatures. The lignin degradation ability was determined by aniline blue method, guaiacol method and calcium lignosulfonate degradation test. Carboxymethylcellulose sodium hydrolyzate loop assay and extracellular enzyme activity determination of cellulose degradation capacity; with weight loss method and vancomycin detergent method to detect its degradation effect on rice straw. 【Result】 The results showed that all tested strains of thermoresistant Dahsiella were able to tolerate high temperature of 50 ° C and could produce cellulase, but different strains produced different ligninolytic enzymes; all had the ability to degrade stalks, of which T. chrysogenum strain H08.1 had the strongest degradation ability, followed by T.cinerea strain H57.1, and its straw degradation rates were 50.2% and 42.2%, respectively. 【Conclusion】 The results showed that there was a potential value for the development and utilization of the straw by the strain of Dahechow-H08.1 and D-strain of Staphylococcus epidermidis H57.1.