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E8启动子是常用的番茄果实特异表达启动子之一,是指番茄E8基因5’侧翼近2.2 kb的DNA序列。前人的研究表明,E8基因5’侧翼-2181--1088区段的删除使E8基因表达量大幅度下降(仅为完整E8启动子的1/10),同时该区段还是E8基因对乙烯应答的充分必要区域;这说明了该区段是E8基因表达调控的重要元件。 为了探究不同遗传型栽培番茄(Lycopersicon esculentum)E8基因的5’侧翼-2181--1088区段DNA序列是否存在差异,作者以F1:5’-cca agc ttg aat tca ttt ttg aca tc-3’和R1:5’-cgc gga tcc cta gat atg ggt ctt tct ag-3’为引物,在pfu
The E8 promoter is one of the commonly used tomato fruit-specific promoters and refers to a DNA sequence approximately 2.2 kb flanking the 5 ’of the tomato E8 gene. Previous studies have shown that deletion of the 5,181-1088 segment of the E8 gene results in a significant decrease in E8 gene expression (only 1/10 of the full E8 promoter), while this segment is also responsible for the E8 gene response to ethylene Sufficient and necessary region of response; this indicates that the segment is an important element of E8 gene expression regulation. In order to investigate whether there is any difference in the DNA sequence of the 5’18 flanking -1881-1088 segment of E8 gene in different genotypes of cultivated tomato (Lycopersicon esculentum), F1: 5’-cca agc ttg aat tca ttt ttg aca tc-3 ’and R1: 5’-cgc gga tcc cta gat atg ggt ctt tct ag-3 ’as primer, at pfu