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利用不同浓度 Na Cl处理高冰草、小麦 ,小麦和高冰草体细胞杂种 F3代 - 1、 - 2的幼苗 ,5d后观察其形态 ,计算耐盐系数 ,测定生长量及脯氨酸含量 ,Na+、K+、Ca2 +等生理指标。结果表明 ,高冰草具有典型耐盐单子叶植物的特征。亲本小麦属非盐生植物 ,抗盐能力较弱 ,当 Na Cl浓度达 0 .5%时已有盐害影响 ,超过后迅速加重甚至死亡 ,而杂种受害的盐度比小麦高。 1 .0 %以上 Na Cl处理组中 ,死亡率远较小麦低 ,生长量及各生理指标的测定也表明杂种较其亲本小麦 (济南 1 77)具有较高的耐盐性。证明通过体细胞杂交高冰草与抗盐有关的某些基因已转入小麦。
The seedlings of somatic hybrids F3, -1, -2 of Agropyron, wheat, wheat and Agropyron brasiliensis were treated with different concentrations of NaCl. After 5 days, the morphology of the seedlings was observed and the salt tolerance coefficient was calculated. The growth and proline content, Na + , K +, Ca2 + and other physiological indicators. The results show that the tall ice grass has the characteristics of a typical salt-tolerant monocotyledon. The parents of non-halophyte Triticum, salt-resistant ability is weak, when the Na Cl concentration of 0.5% has been salt damage, after the rapid increase or even death over, while the victims suffer salinity higher than wheat. More than 1% NaCl in the treatment group, the mortality rate is far lower than the wheat, growth and physiological indicators of the test also showed that hybrids than their parents wheat (Jinan 1 77) has a higher salt tolerance. Proves that some genes related to salt resistance through somatic hybridization have been transferred to wheat.