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目的:采用加校正因子的自身对照法测定淫羊藿饮片中6种成分含量。方法:Agilent ZORBAX Eclipse XDB-C18(150mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水梯度洗脱,检测波长268 nm,柱温30℃。通过加入自身内标物色谱峰峰面积增加值建立校正因子,用相对保留时间和光谱对照法确定其他成分位置,采用HPLC色谱法对其他成分进行定量分析。结果:淫羊藿饮片中朝藿定A、朝藿定A1、朝藿定B、朝藿定C、宝藿苷I相对于淫羊藿苷的保留时间分别为0.750,0.810,0.865,0.939,1.651;校正因子分别为0.998 6,0.998 7,0.998 8,0.989 4,0.985 6。结论:加校正因子的自身对照法可以用于相同类型化学环境成分的定量测定,该方法简便、快捷,适合中药多成分的定量分析。
OBJECTIVE: To determine the contents of six components in Epimedium Pieces by using self-control method with correction factor. METHODS: Agilent ZORBAX Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-water gradient with a detection wavelength of 268 nm and a column temperature of 30 ° C. The calibration factors were established by adding their own internal standard peak-to-peak area-added values. The relative retention time and spectral control method were used to determine the positions of other components. The other components were quantitatively analyzed by HPLC. Results: Epimedium Pieces of Epimedin A, Epimedin A1, Epimedin B, Epimedin C, Poinoside I relative to icariin retention time were 0.750,0.810,0.865,0.939, 1.651; the correction factors were 0.998 6,0.998 7,0.998 8,0.989 4,0.985 6 respectively. Conclusion: The self-control method with correction factor can be used for the quantitative determination of the chemical components of the same type. The method is simple, rapid and suitable for the quantitative analysis of multi-component of traditional Chinese medicine.