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目的:建立高效液相色谱法同时测定蒙药那如三味丸中没食子酸和胡椒碱含量的方法。方法:采用Ultimate XB-C18(250 mm×4.6 mm,5μm)为色谱柱;以甲醇-0.1%磷酸为流动相,梯度洗脱(0~20 min,流动相比例15∶85→80∶20;20~30min,流动相比例80∶20→15∶85),流速为1.0 ml·min-1,检测波长为285 nm,柱温:30℃,进样量:10μl。结果:在此色谱条件下,没食子酸和胡椒碱达到基线分离,且线性良好,线性范围分别为0.878~4.392μg(r=1.000 0)和0.262~1.308μg(r=1.000 0);平均回收率分别为100.75%,RSD=1.19%和98.35%,RSD=1.46%(n=9)。结论:该方法精密度高,分离度好,可为该药的全面质量评价提供参考。
Objective: To establish a method for simultaneous determination of gallic acid and piperine in Mongolian medicine such as Sanwei Pill by high performance liquid chromatography. Methods: The column of Ultimate XB-C18 (250 mm × 4.6 mm, 5 μm) was used as the column. The mobile phase consisted of methanol and 0.1% phosphoric acid with gradient elution (0-20 min, mobile phase 15:85 → 80:20, 20 ~ 30min, mobile phase ratio 80:20 → 15:85), the flow rate was 1.0 ml · min-1, the detection wavelength was 285 nm, the column temperature was 30 ℃ and the injection volume was 10μl. Results: Under the chromatographic conditions, gallic acid and piperine were separated baseline with good linearities of 0.878 ~ 4.392μg (r = 1.000 0) and 0.262 ~ 1.308μg (r = 1.000 0) respectively. The average recoveries Respectively 100.75%, RSD = 1.19% and 98.35%, RSD = 1.46% (n = 9). Conclusion: The method has high precision and good resolution, which can provide a reference for the overall quality evaluation of the drug.