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目的 :改进酸性反流性食管炎大鼠模型 ,统一结扎幽门 ,从而去除因反流量不同而造成的结果偏差。方法 :模型组大鼠行不全幽门结扎加贲门肌切开术。用外径为 1.5 5 m m的金属针穿刺入胃并通过幽门 ,结扎剩余幽门 ,贲门肌纵行切开 0 .5 cm。对照组行全幽门结扎加贲门肌切开术。结果 :模型组与对照组的食管下段粘膜 p H值相比较差异无显著性意义 (P >0 .0 5 )。模型组大鼠均存活 ,存活率为 10 0 %。对照组大鼠死亡 3只 ,存活率为6 2 .5 %。结论 :该模型的技术改进在增加存活率的基础上 ,保证了大鼠模型幽门内径的一致 ,操作上更为规范
OBJECTIVE: To improve the rat model of acid reflux esophagitis and unite the pylorus to remove the deviations caused by the different reflux. Methods: Rats in model group were given incomplete pyloric ligation and cardiac musculotomy. With an outer diameter of 1.5 5 m m of the metal needle puncture into the stomach and through the pylorus, ligation of the remaining pyloric, cardiac muscle longitudinal incision 0.5 cm. The control group with full pyloric ligation plus cardiomyotomy. Results: There was no significant difference in p H value between the model group and the control group in the lower esophageal mucosa (P> 0.05). Rats in model group survived with a survival rate of 100%. Three rats died in the control group, with a survival rate of 62.5%. Conclusion: The technical improvement of this model, on the basis of increasing the survival rate, ensures the consistency of the pylorus inner diameter in the rat model and is more standardized in operation