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在生理酸度条件下,采用紫外光谱和荧光光谱法研究异丙威与小牛胸腺DNA的作用表明:DNA对异丙威的荧光有明显的猝灭作用,属于静态猝灭方式;K4[Fe(CN)6]猝灭试验发现DNA对异丙威有明显的保护作用,离子强度的改变对异丙威和异丙威-DNA体系的荧光均无明显影响;异丙威的加入使DNA的熔点升高,并且异丙威能够竞争置换EB与DNA的结合位点。上述实验也表明,异丙威以嵌插方式作用于DNA的结合位点,有可能通过形成DNA加合物的形式造成DNA损伤,从而最终导致基因突变。
Under the condition of physiological acidity, the effect of isoprenaline and calf thymus DNA by ultraviolet spectroscopy and fluorescence spectroscopy showed that the DNA quenched the fluorescence of isoprocarb significantly and belongs to the static quenching mode. K4 [Fe ( CN) 6] quenching test found that DNA has a significant protective effect against isoprocarb, ionic strength changes have no significant effect on the fluorescence of isoprocarb and isoprocarb-DNA system; the addition of isoprocarb causes the melting point of DNA And that isopiper can compete for the site of substitution of EB for binding to DNA. The above experiment also shows that isoprocarb intercalative effect on the DNA binding sites, DNA adducts may be formed by the form of DNA damage, which eventually led to gene mutations.