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为了探讨西咪替丁 (CMD)对肾综合征出血热 (HFRS)NK细胞及可溶性白细胞介素受体的影响 ,采用流式细胞分析仪检测NK细胞活性 ,可溶性白细胞介素II受体 (SIL -2R)采用夹心酶联免疫吸附法。结果是NK活性及SIL -2R治疗前CMD组与对照组差异无显著性 ;治疗 6d后 ,CMD组及对照组NK活性分别是 (5 9.2 77± 19.819)、(4 1.5 49±15 .40 9)CMD组NK活性明显升高 ,与对照组比较差异有显著性 (P <0 .0 1)。CMD组及对照组SIL -2R分别是 (2 96.4±48.5 )、(4 0 8.6± 69.1) ,CMD组SIL -2R活性明显降低 ,而对照组略有升高 ,CMD抑制SIL -2R活性 ,与对照组比较差异有显著性 (P <0 .0 1)。这表明 ,CMD治疗HFRS的抗病毒作用是通过抑制SIL -2R活性 ,增强NK细胞活性。
To investigate the effect of cimetidine (CMD) on NK cells and soluble interleukin (IL) receptor in hemorrhagic fever with renal syndrome (HFRS), the activity of NK cells, the activity of soluble interleukin II receptor -2R) using sandwich enzyme-linked immunosorbent assay. The results showed that NK activity and SIL-2R before treatment did not differ significantly between the CMD group and the control group; after 6 days of treatment, the NK activity in the CMD group and the control group were (5 9.277 ± 19.819), (4 1.549 ± 15 .409 ) NK activity in CMD group was significantly higher than that in control group (P <0.01). The SIL -2R of CMD group and control group were (2 96.4 ± 48.5) and (40 ± 8.6 ± 69.1) respectively, while the SIL -2R activity of CMD group was significantly lower than that of CMD group The difference between the control group was significant (P <0.01). This indicates that the anti-viral effect of CMD in the treatment of HFRS enhances NK cell activity by inhibiting SIL-2R activity.