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通过辐射聚合,在纸表面覆盖阳离子——疏水性共聚物polo(DEAEMA-ATMPT)和阴离子——疏水性共聚物poly(AA-ATMPT)作为固定化绿色木霉细胞的载体。试验结果表明:用覆盖poly(DEAEMA-ATMPT)的载体固定化细胞比较牢固,未因振荡而解吸;固定化细胞的FPA(滤纸活性)均高于未固定化的游离细胞,其中用覆盖25％:75％ poly(DEAEMA-ATMPT)的载体固定化细胞的FPA比游离细胞增加了20％;固定化细胞的重量随共聚物中阳离子组分的增加而增加。用覆盖poly(AA-ATMPT)的载体固定化绿色木霉也有较好的效果。但固定化细胞的重量明显低于覆盖poly(DEAEMA-ATMPT)的载体固定化细胞的重量,且它随共聚物中阴离子组分的增加而下降。阳离子——疏水性共聚物能提高固定化绿色水霉的效果;用离子型共聚材料固定化细胞静电引力起了主要的作用。
The paper surface was covered by radiation polymerization with the cationic-hydrophobic copolymer polo (DEAEMA-ATMPT) and the anionic-hydrophobic copolymer poly (AA-ATMPT) as carriers for immobilized Trichoderma viride cells. The results showed that the immobilized cells were more stable with poly (DEAEMA-ATMPT) and did not desorption due to oscillation. The immobilized cells had higher FPA (filter paper activity) than the non-immobilized free cells, : The immobilized cells with 75% poly (DEAEMA-ATMPT) had a FPA increase of 20% compared to the free cells; the weight of the immobilized cells increased with the increase of the cationic components in the copolymer. Trichoderma viride immobilized with a poly (AA-ATMPT) -based vector also had good results. However, the weight of the immobilized cells was significantly lower than that of the vector-immobilized cells covered with poly (DEAEMA-ATMPT), and it decreased with the increase of anionic components in the copolymer. Cationic-hydrophobic copolymers can improve the immobilization of green mold; the electrostatic attraction of immobilized cells with ionic copolymeric materials plays a major role.