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甘蓝型油菜细胞核雄性不育是杂种优势利用的重要途径。隐性上位互作核不育系9012A已经广泛用于杂交种子生产,其不育性受两对隐性重叠不育基因(ms1和ms2)与一对隐性上位抑制基因(rf)互作控制。ms1和ms2同时纯合(ms1ms1ms2ms2)表现不育,但隐性纯合rf(rfrf)对ms1ms1ms2ms2的表达起抑制作用,又可使其表现可育(TAM系,ms1ms1ms2ms2rfrf)。本研究利用AFLP和SRAP分子标记技术,分别筛选了884对AFLP引物和506对SRAP引物,筛选到了14个与不育基因Ms1基因紧密连锁的标记,其中4个标记与育性共分离。Ke等发表的AFLP标记E-ACA/P-CTG在本研究群体中与Ms1基因相距0.1cM。紧密连锁标记测序序列BLASTN结果表明,其和拟南芥第五染色体高度同源,并且通过和已测序的甘蓝C基因组序列进行比对,将其定位在C9染色体的末端。本研究结果有助于该基因的分子标记辅助选择和克隆。本文还对前人发表的与Ms1基因连锁的标记在本研究群体中的分离情况作了探讨。
Male Sterility in Brassica napus is an Important Way to Utilize Heterosis. Recessive upper male sterile line 9012A has been widely used in hybrid seed production and its sterility is controlled by two pairs of recessive overlapping sterility genes (ms1 and ms2) and a pair of recessive upper inhibitory genes (rf) . ms1 and ms2 are both homozygous (ms1ms1ms2ms2), but implicit homozygous rf (rfrf) can inhibit the expression of ms1ms1ms2ms2 and make it fertile (TAM, ms1ms1ms2ms2rfrf). In this study, 884 pairs of AFLP primers and 506 pairs of SRAP primers were screened using AFLP and SRAP markers, respectively, and 14 markers closely linked to the Ms1 gene of sterile gene were screened. Among them, 4 markers were separated from fertility. The AFLP marker E-ACA / P-CTG published by Ke et al. Is 0.1 cM away from the Ms1 gene in this study population. The BLASTN sequence of the closely linked marker sequence showed that it was highly homologous to the fifth chromosome of Arabidopsis thaliana, and was mapped to the C terminus of C9 genome by aligning with the sequenced C genome sequence. The results of this study contribute to the molecular marker-assisted selection and cloning of this gene. This article also discussed the isolation of the markers linked to Ms1 gene published in previous study in this study population.