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目的对赤道几内亚Bioko岛上恶性疟原虫多药抗性基因(pfMDR-1)进行分析,为Bioko岛的疟疾防控和治疗提供依据。方法2012年雨季期间采集的恶性疟原虫感染患者样本151份,用巢式PCR技术特异性扩增N86Y、E130K、Y184F、S1034C、N1042D、V1109I、D1246Y耐药分子标记的pfMDR-1基因片段,然后进行测序分析。结果虫株中共发现了4种不同的单倍型:YEY/SNVD、NEF/SNVD、YEF/SNVD和NEY/SNVD。91.39%(138/151)的样本发现了耐药性位点突变,包括3.31%(5/151)的86Y,29.80%的184F,和58.29%(88/151)的双重突变86Y/184F。结论结果表明赤道几内亚Bioko岛上的恶性疟原虫株存在较高比例耐药基因突变和耐药复合基因突变,为当地的抗疟疾选用药物提供指导。
Objective To analyze the pfMDR-1 gene of Plasmodium falciparum on Bioko Island in Equatorial Guinea, and provide basis for the prevention and treatment of malaria on Bioko island. Methods 151 samples of P. falciparum infection collected during the rainy season in 2012 were used to amplify the pfMDR-1 gene fragment of N86Y, E130K, Y184F, S1034C, N1042D, V1109I and D1246Y resistant cells by nested PCR. Perform sequencing analysis. Results Four different haplotypes were found in the strains: YEY / SNVD, NEF / SNVD, YEF / SNVD and NEY / SNVD. A total of 91.39% (138/151) of the samples identified resistance site mutations including 86Y, 29.80% 184F and 58.29% (88/151) double mutation 86Y / 184F of 3.31% (5/151). Conclusion The results showed that the P. falciparum strain on Bioko Island in Equatorial Guinea has a high proportion of resistance gene mutations and drug-resistance gene mutations, providing guidance for local anti-malaria drug selection.