使用砷钼酸比色法,以葡萄糖为标准品,在波长560nm处测吸光度值求得粗多糖含量。葡萄糖含量在0.02mg-0.10mg范围内与吸光度值呈线性关系,回归方程Y=5.845X-0.072 1,相关系数r=0.999 3,平均回收率为96.42%,相对标准差RSD=1.565%(n=5)。砷钼酸比色法方法易于操作,反应产物稳定,测试结果准确度高、重现性好,是测定保健酒及其它保健食品中粗多糖含量的理想方法。 Arsenic molybdate colorimetric method to glucose as a standard, measured at 560nm wavelength absorbance value obtained crude polysaccharide content. The correlation coefficient r = 0.999 3, the average recovery was 96.42%, the relative standard deviation RSD was 1.565% (n = 5). Arsenomolytic acid colorimetric method is easy to operate, the reaction product is stable, the test results with high accuracy and good reproducibility, is an ideal method for the determination of crude polysaccharide in health wine and other health food.