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目的:探讨健脾化瘀方含药血清对人结肠癌细胞LoVo凋亡的影响。方法:①应用MTT法检测健脾化瘀方含药血清对人结肠癌细胞LoVo的增殖抑制作用;②运用TUNEL法和AnnexinV/PI法检测LoVo细胞的凋亡率。结果:①健脾化瘀方含药血清高浓度(20%)可抑制LoVo细胞的增殖(P<0.01);②健脾化瘀方含药血清作用细胞48h后可以诱导细胞凋亡。结论:健脾化瘀方含药血清能抑制结肠癌细胞LoVo的增殖能力,并诱导细胞凋亡。
Objective: To investigate the effect of Jianpi Huayu Recipe containing serum on the apoptosis of LoVo human colon cancer cells. Methods: ①MTT method was used to detect the effect of Jianpi Huayu Recipe serum on the proliferation of human colon cancer cell LoVo; ②The apoptosis rate of LoVo cells was detected by TUNEL and AnnexinV / PI. Results: ① The drug-containing serum of Jianpi Huayu Fang high concentration (20%) can inhibit the proliferation of LoVo cells (P <0.01); ② The effect of Jianpi Huayu Recipe containing serum can induce cell apoptosis after 48 hours. Conclusion: Jianpi Huayu Fang containing serum can inhibit the proliferation of colon cancer cells LoVo and induce apoptosis.