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用焦锑酸钾沉淀法进行了组织和细胞中游离钙的化学定位。用光学显微镜和透射电镜观察石刁柏幼苗在太空飞行后Ca2+沉淀颗粒在根尖组织和细胞内的分布。结果表明,太空飞行15天后,Ca2+在各组织内的分布情况与地面对照无明显差异,但Ca2+的含量明显低于对照。Ca2+在细胞内不同区域的分布在飞行和对照样品中差异十分明显,对照细胞中Ca2+集中在液泡内,其它细胞器中很少见到。飞行幼苗的根尖细胞,液泡中Ca2+很少,并向液泡膜集结,液泡膜内侧和细胞质中的Ca2+明显增多。细胞壁中的Ca2+较对照有明显增加,高尔基体中也有少量钙存在。本文着重讨论了飞行幼苗根尖中Ca2+在细胞内重新分布的可能作用。
The chemical localization of free calcium in tissues and cells was performed using potassium pyroantimonate precipitation method. The distribution of Ca2 + precipitated particles in apical tissues and cells after astronautic flight was observed by light microscopy and transmission electron microscopy. The results showed that after 15 days of space flight, the distribution of Ca2 + in each tissue had no significant difference with the ground control, but the content of Ca2 + was obviously lower than that of the control. The distribution of Ca2 + in different regions of the cell is very different in flight and control samples. Ca2 + in control cells is concentrated in the vacuole, which is seldom seen in other organelles. The root tip cells of flying seedlings had less Ca2 + in the vacuoles and accumulated to the vacuole membrane, and Ca2 + in the medial and cytoplasm of the vacuole membrane increased significantly. Ca2 + in the cell wall increased significantly compared with the control, and there was also a small amount of calcium in the Golgi apparatus. This article focuses on the possible role of intracellular Ca2 + redistribution in the root tips of flying seedlings.