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目的:研究血管紧张素转化酶(ACE)基因多态性在海南黎、汉族原发性高血压(EH)人群的频率分布差异。方法:采用聚合酶链反应(PCR)方法,检测海南黎族111例EH患者(黎族组),106例汉族EH患者(汉族组)ACE基因多态性,观察DD、DI、II基因型频率及等位基因频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率。结果:黎族组DD、DI、II基因型频率分别为10.8%、37.8%、51.4%,D及I等位基因频率分别为29.7%、70.3%;汉族组DD、DI、II基因频率为16.0%、28.3%、55.7%,D及I等位基因分别为30.2%、69.8%,2组比较,差异无统计学意义。结论:ACE基因多态性不是海南黎族EH人群发病率不同于汉族人群的主要原因。
Objective: To study the frequency distribution of angiotensin-converting enzyme (ACE) gene polymorphism in essential hypertension (EH) population of Li and Han nationalities in Hainan Province. Methods: Polymerase chain reaction (PCR) was used to detect ACE gene polymorphisms in 111 cases of EH patients (Li group) and 106 cases of EH patients (Han group) of Hainan Li ethnic group. The frequencies of DD, DI and II genotypes were observed Bit gene frequency, and all the ordinary PCR set as DD-type samples insert specific PCR detection to reduce the error rate. Results: The frequencies of DD, DI, and II genotypes in Li group were respectively 10.8%, 37.8% and 51.4%, and the frequencies of D and I alleles were 29.7% and 70.3% respectively. The frequencies of DD, DI and II genes in Han nationality were 16.0% , 28.3%, 55.7% respectively. The D and I alleles were 30.2% and 69.8% respectively. There was no significant difference between the two groups. Conclusion: The ACE gene polymorphism is not the main reason why the incidence of EH in Hainan Li ethnic group is different from that in Han population.