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目的探索潮州某驻军登革热和流行性乙型脑炎(乙脑)等虫媒病综合防制效果,为预防和控制虫媒病毒病提供科学依据。方法收集发热可疑患者和健康人血标本及蚊虫标本作病毒分离和血清学抗体调查;按全面或随机法调查营区内蚊虫孳生情况,人诱法监测成蚊叮刺率;用C6/36细胞培养法分离病毒并用间接免疫荧光法(IFAT)或逆转录聚合酶链反应(RT PCR)法鉴定;用IFAT调查健康人群登革热和乙脑抗体;以“治本清源”为原则,普及防病知识和开展爱国卫生运动,针对不同孳生地类型和栖息特点采用不同方法处理。结果经监测发热待查病人和媒介蚊虫均未分离出病毒;健康人群登革热和乙脑的抗体水平不高;处理前蚊类优势种为白纹伊蚊和致倦库蚊,有少量三带喙库蚊和骚扰阿蚊,其中白纹伊蚊的容器指数(CI)为24.1,百户指数(BI)为30.0,均高于规定的危险地区界限20.0。结论该驻地存在登革热和乙脑暴发流行潜在危险,蚊虫密度较高,人群抗体水平低,采用综合防制措施后,经3年连续观察,蚊媒已控制在不足为害的水平。
Objective To explore the integrated control effect of arbovirus such as dengue fever and Japanese encephalitis (JE) in a certain place in Chaozhou and provide a scientific basis for the prevention and control of arbovirus disease. Methods Blood samples and mosquitoes samples of feverish people and healthy people were collected for virus isolation and serological antibody investigation. Mosquito breeding in the camp area was investigated by comprehensive or random methods. The virus was isolated and identified by indirect immunofluorescence (IFAT) or reverse transcriptase-polymerase chain reaction (RT PCR). IFAT was used to investigate dengue and JE antibody in healthy population. Patriotic health campaigns should be carried out to deal with different types of breeding habitats and habitats. Results The virus was not isolated from the fever patients and the vector mosquitoes. The antibody levels of dengue and JE in healthy people were not high. The dominant mosquito species before treatment were Aedes albopictus and Culex pipiens quinquefasciatus, The mosquitoes and mosquitoes were mosquitoes, of which Aedes albopictus had a container index (CI) of 24.1 and a BI index of 30.0, both of which were higher than the limit of 20.0 for the dangerous areas. Conclusion The resident has the potential danger of Dengue Fever and JE outbreak. The density of mosquitoes is high and the population antibody level is low. After three years of comprehensive control measures, the mosquito vector has been controlled at a level less than adequate.