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17β-雌二醇(E_2)和孕酮(P)相互作用调节着哺乳类子宫结构和机能的周期性变化。近年来有些学者通过观察受体的激素调节,对子宫内E_2和P交互作用的分子基础进行了研究,他们发现,E_2可以刺激E_2和P胞浆受体的合成,而P拮抗雌激素的作用,其机理可能是由于P抑制E_2胞浆受体的合成(或补充)。本实验观察了在体条件下P对田鼠子宫的核部分和胞浆部分摄取和保留~3H-E_2的作用,同时用小肠作为对照组织。成年雌性田鼠,于动情间期切除卵巢,皮下埋植E_2以维持循环中E_2处于较高的生理浓度。24小时后除去埋植的E_2,或皮下注射~3H-E_2同时皮下注射P。或皮下注射~3H-E_2同时注射玉米油。注药后
The interaction of 17β-estradiol (E_2) and progesterone (P) regulates the periodic changes in the structure and function of mammalian uterus. In recent years, some scholars have studied the molecular basis of intrauterine E 2 and P interaction by observing the hormone regulation of the receptors. They found that E 2 can stimulate the synthesis of E 2 and P cytoplasmic receptors, while P antagonizes the effect of estrogen , The mechanism may be due to P inhibition of E 2 cytosolic receptor synthesis (or supplement). In this experiment, we observed the uptake and retention of ~ 3H-E_2 in the nuclear and cytoplasmic fractions of the uterus in vitro under the condition of P, while the small intestine was used as control. Adult female vole, ovariectomized during estrus, subcutaneously implanted E 2 to maintain the cycle of E 2 at a high physiological concentration. After 24 hours, the embedded E 2 was removed, or ~ 3H-E 2 subcutaneously injected subcutaneously at the same time. Or subcutaneous injection ~ 3H-E_2 corn oil simultaneously. After injection