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目的:研究非小细胞肺癌微转移与微血管密度的特点,分析肿瘤的脉管内侵犯与微转移、微血管密度之间的关系。方法:采用免疫组化染色方法应用CD34单抗检测47例肿瘤组织的脉管内侵犯及微血管密度;采用RT-PCR技术检测47例患者外周血中CK19 mRNA的表达。结果:47例患者中18例有脉管内侵犯,其平均微血管密度为29.3±3.3,高于非脉管内侵犯的平均微血管密度25.2±4.7(P<0.05);18例有脉管内侵犯病例中,有13例CK19 mRNA阳性,5例阴性,29例无脉管内侵犯病例中,9例CK19 mRNA阳性,20例阴性,具有显著差异性(P<0.05)。脉管内侵犯与年龄、肿瘤大小、分化程度无关(P>0.05),而与肿瘤的胸膜浸润、淋巴结转移及远处转移密切相关(P<0.05)。结论:非小细胞肺癌的脉管内侵犯与微转移、微血管密度之间的关系密切,是评估肿瘤生物学行为的重要临床病理特征。
Objective: To study the characteristics of micrometastasis and microvessel density in non-small cell lung cancer (NSCLC) and to analyze the relationship between intravascular invasion and micrometastasis and microvessel density. Methods: Immunohistochemical staining was used to detect the invasion and microvessel density of 47 tumor tissues by CD34 monoclonal antibody. The expression of CK19 mRNA in 47 patients was detected by RT-PCR. RESULTS: Eighteen of 47 patients had vascular invasion, the mean microvessel density was 29.3 ± 3.3, which was higher than that of noninvasive invasive microvessel density 25.2 ± 4.7 (P <0.05). Among the 18 cases with intravascular invasion, CK19 mRNA was positive in 13 cases and negative in 5 cases. Of the 29 cases without vascular invasion, 9 cases were positive for CK19 mRNA and 20 cases were negative, with significant difference (P <0.05). Vascular invasion was not related to age, tumor size and differentiation (P> 0.05), but was closely related to pleural invasion, lymph node metastasis and distant metastasis (P <0.05). Conclusion: There is a close relationship between vascular invasion and micro-metastasis and microvessel density in non-small cell lung cancer. It is an important clinical and pathological feature to evaluate the biological behavior of non-small cell lung cancer.