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背景与目的本课题组前期研究提示CD24可促进大肠癌细胞的增殖,同时可以激活Raf-ERK通路和p38MAPK激酶。但CD24分子因缺乏跨膜结构而不足以传递细胞信号,而Src家族激酶和MAPK通路关系密切且和肠癌密切相关。因此,本研究拟探讨结直肠癌组织中CD24及SFKs成员之一的Src的表达和相互关系,及其在结直肠癌演进过程中的作用,为进一步的功能鉴定奠定基础。方法对204例结直肠癌组织进行连续切片,应用免疫组化方法检测CD24、Src的表达情况,分析两者表达的相关性及与结直肠癌患者临床病理特征和预后的关系。结果免疫组化染色提示,正常结直肠黏膜组织中CD24、Src均无表达,而在结直肠癌组织中则有不同程度的CD24、Src染色。二者的表达强弱情况均与肿瘤细胞的分化程度、Duke分期、肿瘤浸润程度、淋巴结转移以及远处转移有关(P<0.01),而与患者年龄、性别、肿瘤部位以及肿瘤大小无关(P>0.05)。相关分析统计提示CD24表达与Src表达呈正相关关系(r=0.544,P<0.01)。生存分析提示CD24及Src低表达患者的生存率均要高于高表达患者;COX模型多因素分析显示,CD24和Src表达不是独立的预后因素。结论 CD24和Src的表达与结直肠癌的演进密切相关,二者组织学表达具有相关性,CD24和Src的表达不是结直肠癌的独立预后因素。
Background and Objective Our previous study suggested that CD24 may promote the proliferation of colorectal cancer cells and activate the Raf-ERK pathway and p38 MAPK kinase at the same time. But CD24 molecule is not enough to transmit cell signal due to the lack of transmembrane structure. Src family of kinases and MAPK pathway are closely related and are closely related to intestinal cancer. Therefore, this study was to investigate the expression and correlation of Src, a member of CD24 and SFKs, in colorectal cancer tissues and its role in the process of colorectal cancer evolution, and lay the foundation for further functional identification. Methods Totally 204 cases of colorectal cancer tissues were sectioned. The expressions of CD24 and Src were detected by immunohistochemistry. The correlation between them and the clinicopathological features and prognosis of colorectal cancer were analyzed. Results Immunohistochemical staining showed no expression of CD24 and Src in normal colorectal mucosa, but different degrees of CD24 and Src staining in colorectal cancer tissues. The expression of both was related to the degree of tumor differentiation, Duke stage, degree of tumor infiltration, lymph node metastasis and distant metastasis (P <0.01), but not with the age, sex, tumor location and tumor size > 0.05). Correlation analysis showed that there was a positive correlation between CD24 expression and Src expression (r = 0.544, P <0.01). Survival analysis indicated that the survival rates of CD24 and Src low expression patients were higher than those of high expression patients. Multivariate analysis of COX model showed that CD24 and Src expression were not independent prognostic factors. Conclusion The expressions of CD24 and Src are closely related to the evolution of colorectal cancer. The expression of CD24 and Src is not independent prognostic factor for colorectal cancer.