论文部分内容阅读
目的 探讨重症肌无力 (MG)胸腺微环境内树突状细胞、淋巴细胞和上皮细胞的免疫组织学变化。方法 (1) 2 5例MG病人 (胸腺滤泡性增生者 13例 ,无增生者 12例 )、7例先天性心脏病和 1例新生儿胸腺组织 ,常规石蜡包埋切片 ,HE染色 ,光镜观察。 (2 )免疫化学标记S 10 0、CD1a、CD4、CD8、CD 2 0、CD4 5RO、细胞角蛋白 (CKPan)、上皮细胞膜抗原 (EMA)和癌胚抗原 (CEA) ,光镜观察 ,对其中S 10 0 ,CD1a阳性的树突状细胞半定量计数 ,t检验 ,统计分析。结果 (1)在胸腺淋巴滤泡性增生组和非增生组 ,胸腺单位面积内 (10 0mm2 )表达S 10 0阳性的树突状细胞数是 2 3.5和 4 7.5 ,CD1a为 2 .1和 3.8,两组t检验 ,P <0 .0 5。 (2 )胸腺皮质细胞和淋巴滤泡性增生的髓质分别表达CD1a、CD8和CD4 5RO ,胸腺小体、血管周围淋巴细胞极少表达CD1a ,胸腺髓质和生发中心内淋巴细胞表达CD2 0。 (3)胸腺被膜下、皮质、胸腺小体和髓质内有表达CKPan的上皮细胞 ,但EMA和CEA阴性。结论 (1)MG时胸腺髓质无论有无淋巴滤泡性增生 ,都表达多量抑制T细胞和CD2 0淋巴细胞 ;生发中心内有多量表达CD2 0和少量CD8细胞。 (2 )MG时胸腺微环境内这些抗体的表达和树突状细胞数量的变化 ,将影响T、B淋巴细胞增生和淋巴滤泡形成 ;胸腺内树突状细?
Objective To investigate the immunohistochemical changes of dendritic cells, lymphocytes and epithelial cells in myasthenia gravis (MG) thymus microenvironment. Methods (1) Twenty-five patients with MG (13 cases of thymus follicular hyperplasia, 12 cases without proliferative), 7 cases of congenital heart disease and 1 case of neonatal thymus were paraffin-embedded sections, HE staining, light Mirror observation. (2) The immunochemical markers S 10 0, CD 1 a, CD4, CD 8, CD 2 0, CD4 5RO, CKPan, EMA and CEA were observed by light microscopy. S 10 0, CD1a-positive dendritic cells semi-quantitative counting, t test, statistical analysis. Results (1) In thymus lymphoma follicular hyperplasia group and non-hyperplastic group, the number of S 10 0 positive dendritic cells per unit area (10 0mm2) in thymus was 23.5 and 47.5, while CD1a was 2.1 and 3.8 , T test two groups, P <0. (2) CD1a, CD8 and CD4 5RO were expressed in the medulla of thymus and lymphoid follicular hyperplasia, CD1a was rarely expressed in thymus and perivascular lymphocytes, and CD20 was expressed in lymphocytes in thymus medulla and germinal center. (3) CKPan-expressing epithelial cells in the thymus under the capsule, cortex, thymus and medulla, but EMA and CEA negative. Conclusions (1) MG thymus medulla with or without lymphoid follicular hyperplasia, both expressed a large number of inhibitory T cells and CD20 lymphocytes; a large number of germinal center expression of CD20 and a small amount of CD8 cells. (2) The expression of these antibodies and the number of dendritic cells in the thymus microenvironment during MG affect T, B lymphocyte proliferation and lymphoid follicle formation; dendritic thinning in the thymus