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目的:观察十八反中部分禁忌中药(甘草反甘遂、大戟、海藻、芫花)同用后对大鼠心、肝、肾的毒副反应。方法:采用Wistar大鼠,分为单味药组和配伍药组,每组各10只,雌雄各半。单味药甘草100g加水200mL,煎煮浓缩至50mL药液,配伍组用甘草与甘遂、甘草与大戟、甘草与海藻、甘草与芫花各100g,分别加水400mL,煎煮浓缩至50mL药液。灌胃给药,用药7d后检测肝功、肾功及心肌酶谱,同时作肝脏、肾脏及心脏组织病理切片。结果:甘草与甘遂、海藻配伍组谷丙转氨酶(alaninetransaminase,ALT)犤(4.48±0.43),(4.29±0.42)μkat/L犦较单味药组犤(0.74±0.07),(0.70±0.07)μkat/L〗升高(t=85.42,85.11,P<0.01)。甘草与甘遂配伍组肌酸磷酸激酶(creatinephosphokinase,CPK)、乳酸脱氢酶(lacticdehydrogenase,LDH)比单味药组升高(t=66.19,P<0.01;t=33.64,P<0.05)。甘草与大戟配伍组CPK,LDH、γ-羟丁酸脱氢酶(γ-hydroxybutyratedehydrogenase,γ-HBDH)较单味药组高(t=91.08,76.19,55.65,P<0.05～0.01)。甘草与海藻配伍组CPK,LDH较单味药组高(t=85.73,P<0.01;t=51.14,P<0.05)。甘草与芫花配伍组CPK,LDH较单味药组高(t=66.35,P<0.01;t=31.27,P<0.05)。结论:甘草与甘遂、大戟、海藻、芫花药物配伍后对大鼠心肝肾有一定毒副作用。
OBJECTIVE: To observe the toxic and side effects of the traditional Chinese medicine (Licorice, Anti-kandan, Daxue, Seaweed, Flos Sophorae) on the heart, liver and kidney of rats after 18 years of use. Methods: Wistar rats were divided into single-drug group and compatibility group. Each group had 10 males and females. Single herb Licorice 100g water 200mL, decoction concentrated to 50mL liquid, compatibility group with licorice and kan gan, licorice and daggar, licorice and seaweed, licorice and medlar each 100g, respectively, add water 400mL, decoction concentrated to 50mL drug liquid. After intragastric administration, liver function, renal function, and myocardial enzymes were measured 7 days after the administration, and liver, kidney, and heart tissue sections were also performed. RESULTS: The concentration of alanine transaminase (ALT) 甘 (4.48±0.43) and (4.29±0.42)μkat/L 甘 of licorice with kansui and seaweed were higher than that of singly 药(0.74±0.07) (0.70±0.07). )μkat/L increased (t=85.42, 85.11, P<0.01). The concentrations of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) in the combination of licorice and kansui were higher than those of the saponin group (t=66.19, P<0.01; t=33.64, P<0.05). The concentrations of CPK, LDH and γ-hydroxybutyrated hydrogenohydrogenase (γ-HBDH) in the combination of licorice and Daqian were higher than those in the single-flavored group (t=91.08, 76.19, 55.65, P<0.05-0.01). The CPK and LDH levels in the combination of licorice and seaweed were higher than those in the single herb group (t=85.73, P<0.01; t=51.14, P<0.05). The CPK and LDH of the combination of licorice root and alfalfa flower were higher than that of the single-flavored drug group (t=66.35, P<0.01; t=31.27, P<0.05). Conclusion: Glycyrrhiza uralensis with Ganzi, Daxue, seaweed, and coriander drugs have certain toxic and side effects on rat heart, liver and kidney.