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目的探讨C-erbB-2在口腔鳞状细胞癌(OSCC)中的蛋白表达及基因扩增,并评估其在OSCC发生、发展和预后中的价值。方法收集2003年1月至2013年6月辽宁省人民医院手术切除并经病理确诊为OSCC的石蜡包埋组织蜡块60例及正常口腔黏膜组织蜡块30例,分别采用免疫组织化学SP法与荧光原位杂交(FISH)技术检测C-erbB-2蛋白表达及基因扩增。结果 C-erbB-2蛋白在OSCC、正常口腔黏膜组织中的表达阳性率分别为31.7%、3.3%;扩增阳性率分别为28.3%、0;OSCC中C-erbB-2蛋白表达、基因扩增与正常口腔黏膜组织相比较,差异具有统计学意义(P<0.05);在OSCC组织中C-erbB-2蛋白表达、基因扩增与患者的年龄、性别、肿瘤大小、分化程度均无相关性(均P>0.05),与肿瘤淋巴结转移、远处转移、临床分期相关(χ2=10.808、17.100、4.627,χ2=8.840、20.044、5.102,均P<0.05)。结论 C-erbB-2与OSCC的发生、发展有关,可以将其作为判断OSCC的生物学行为的重要参考指标。
Objective To investigate the protein expression and gene amplification of C-erbB-2 in oral squamous cell carcinoma (OSCC) and to evaluate its value in the occurrence, development and prognosis of OSCC. Methods 60 cases of paraffin embedded tissue paraffin blocks and 30 normal oral mucosal tissue paraffin blocks were collected from Liaoning Provincial People’s Hospital from January 2003 to June 2013. The immunohistochemical SP method and Fluorescence in situ hybridization (FISH) was used to detect C-erbB-2 protein expression and gene amplification. Results The positive rates of C-erbB-2 protein expression in OSCC and normal oral mucosa tissues were 31.7% and 3.3%, respectively. The positive rates of amplification were 28.3% and 0 respectively. The expression of C-erbB-2 protein in OSCC and gene amplification Compared with normal oral mucosa, the difference was statistically significant (P <0.05). There was no correlation between the expression of C-erbB-2 protein and the gene amplification in age, sex, tumor size and differentiation of OSCC (Χ2 = 10.808, 17.100, 4.627, χ2 = 8.840, 20.044, 5.102, all P <0.05). Conclusion C-erbB-2 is related to the occurrence and development of OSCC, which can be used as an important reference to judge the biological behavior of OSCC.