论文部分内容阅读
目的 观察神经损伤后外源性表皮生长因子 (EGF)对神经元保护的情况。方法 雄性SD大鼠18只 ,体重 180～ 2 2 0 g ,随机分成对照组和EGF组 ,每组 9只。将大鼠左侧坐骨神经在梨状肌下缘 0 .5cm处切断 ,近侧端双重结扎。对照组于神经结扎近端注入生理盐水 5 μl、EGF组注入hEGF5 μl( 10 μgEGF溶于 5 μl生理盐水中 )。分别于手术后 7、14、2 8d取L4 6脊髓及L5背根神经节作苏木精 伊红染色及焦油紫染色后计算脊髓内神经元的数目 ,TUNEL标记检测 ,标记凋亡的神经元数目。结果 各时间组的神经元数目 ,EGF组明显多于对照组 (P <0 .0 1)。凋亡细胞在术后 7d、14d时 ,EGF组少于对照组 (P <0 .0 5 ) ,在 2 8d时两组差异无显著性 (P >0 .0 5 )。结论 EGF对神经损伤后相应的神经元有一定的保护作用
Objective To observe the protective effects of exogenous epidermal growth factor (EGF) on neurons after nerve injury. Methods 18 male SD rats weighing 180-220 g were randomly divided into control group and EGF group, with 9 rats in each group. The left sciatic nerve of rats was cut off at 0.5 cm from the inferior edge of piriformis, and the proximal end was double ligation. In the control group, 5 μl of normal saline was injected into the proximal nerve ligation, and 5 μg of hEGF was injected into the EGF group (10 μg of EGF was dissolved in 5 μl of normal saline). L4 6 spinal cord and L5 dorsal root ganglia were harvested at 7, 14, and 28 days after operation for hematoxylin and eosin staining, respectively. The number of neurons in the spinal cord was calculated by TUNEL staining and apoptotic neurons number. Results The number of neurons in each time group was significantly higher than that in the EGF group (P <0.01). The number of apoptotic cells in the EGF group was less than that in the control group at 7d and 14d after operation (P <0.05). There was no significant difference between the two groups at 28d (P> 0.05). Conclusion EGF can protect the corresponding neurons after nerve injury