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采用ＳＤＳ－聚丙烯酰胺凝胶电泳及双向电泳技术对小麦抗、感品系在接种褐斑病菌（Ｐｔｒ）８６－１２４小种后２４，４８，７２ｈ叶片细胞间洗脱液的蛋白质进行了系统的动态研究。结果表明，寄主植物在受到病原物侵染后，２２种病原相关蛋白（ＰＲ）被诱导合成，接种后４８ｈ，ＰＲ蛋白的表达量达最大值，７２ｈ后相应减少。其中２０种组分在抗、感品系间的表达动态没有差异。Ｗｅｓｔｅｒｎ杂交分析证明，其中有７种为β－１，３－葡聚糖酶，４种为几丁酶，１种为ＰＲ－１蛋白，属非特异性抗性反应。研究还发现ｐＩ５．２、２２ｋｄ和ｐＩ６．６、１９ｋｄ的两种蛋白仅在感病品系内被诱导合成，与特异抗性反应有关。从凝胶中回收了这两种组分，并完成其氨基酸序列分析。
SDS-polyacrylamide gel electrophoresis and two-dimensional electrophoresis were performed on the wheat anti-susceptible lines at 24, 48 and 72 h after inoculation with Ptr 86-124. Dynamic research. The results showed that 22 pathogen-related proteins (PRs) were induced from host plants after being infected with pathogens. The expression of PR protein reached its peak at 48h after inoculation and decreased correspondingly after 72h. There was no difference in the expression of 20 components in the anti-sense and susceptible strains. Western blot analysis showed that seven of them were β-1,3-glucanase, four were chitinase and one was PR-1 protein, which was a non-specific resistance reaction. The study also found that pI5.2, 22kd and pI6.6, 19kd two proteins were induced in the susceptible strain synthesis, and specific resistance response. Both components were recovered from the gel and their amino acid sequence analysis was completed.