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目的探讨结肠癌患者外周血CD_4~+CD_(25)~+调节性T细胞检测的临床意义。方法采用流式细胞技术对51例结肠癌患者和30例健康体检者外周血淋巴细胞CD_4及CD_(25)进行了检测。结果(1)结肠癌组CD_4~+CD_(25)~+细胞明显高于正常对照组(P＜0．05),CD_4~+CD_(25)~-细胞则显著低于正常对照组(P＜0．05)；(2)结肠癌组手术后患者CD_4~+CD_(25)~+、CD_4~-CD_(25)~+细胞与手术前相比显著下降(P＜0．05)；(3)结肠癌组淋巴结转移患者CD_4~+CD_(25)~+细胞明显高于未转移组(P＜0．001)。结论CD_4~+CD_(25)~+调节性T细胞数可以反映肿瘤患者免疫系统存在抑制状态,且可能与肿瘤的免疫耐受有关,并可反映肿瘤淋巴结转移状况。
Objective To investigate the clinical significance of detection of CD_4 ~ + CD_ (25) ~ + regulatory T cells in peripheral blood of patients with colon cancer. Methods Flow cytometry was used to detect the CD_4 and CD_ (25) of peripheral blood lymphocytes in 51 patients with colon cancer and 30 healthy controls. Results CD_4 ~ + CD_ (25) ~ + cells in colon cancer group were significantly higher than those in normal control group (P <0.05), and CD_4 ~ + CD_ (25) 0.05). (2) The numbers of CD_4 ~ + CD_ (25) ~ +, CD_4 ~ -CD_ (25) ~ + cells in colon cancer patients after operation decreased significantly compared with those before operation (P < (3) CD_4 ~ + CD_ (25) ~ + cells in colon cancer patients with lymph node metastasis were significantly higher than those without metastasis (P <0.001). Conclusions The number of CD_4 ~ + CD_ (25) ~ + regulatory T cells can reflect the existence of the immune system in tumor patients, and may be related to the immune tolerance of the tumor and may reflect the status of tumor lymph node metastasis.