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目的了解2008年广西一起甲型肝炎暴发流行株基因型。方法采自甲型肝炎暴发流行中3所学校现症病人的血清,经RT-PCR对HAV-VP1区核苷酸进行扩增和序列分析。结果来自3所学校的HAV流行株VP1区核苷酸序列同源性为100%,与AH2株的同源性最高,为98.6%,同属于IA亚型;氨基酸水平上没有发生氨基酸的缺失、插入,未发生有意义的突变。结论IA亚型是导致此次甲肝暴发的流行株,3所学校具有相同的传染源和流行株。人群甲肝疫苗免疫水平低是甲肝流行的主要因素,应加强甲肝疫苗的免疫水平及甲肝分子流行病学监测。
Objective To understand the genotype of a pandemic strain of hepatitis A in Guangxi in 2008. Methods The sera from three school-based patients with hepatitis A outbreak were collected. The nucleotide sequence of HAV-VP1 region was amplified by RT-PCR and sequenced. Results The homology of nucleotide sequence of VP1 of HAV epidemic strains from three schools was 100%, the highest homology with AH2 strain was 98.6%, belonging to IA subtype. There was no amino acid deletion in amino acid level, Inserted, no meaningful mutation occurred. Conclusion The subtype IA is the epidemic strain leading to the outbreak of hepatitis A, and the three schools have the same infectious and epidemic strains. People with low levels of hepatitis A vaccine is a major factor in the epidemic of hepatitis A, hepatitis A vaccine should be strengthened and hepatitis A molecular epidemiological surveillance should be strengthened.