论文部分内容阅读
为比较斑点免疫金银染色（Ｄｏｔ－ＩＧＳＳ）和Ｄｏｔ－ＥＬＩＳＡ检测弓形虫抗体的敏感性和特异性，本实验以弓形虫速殖子可溶性蛋白抗原为诊断用抗原，同时用Ｄｏｔ－ＩＧＳＳ和Ｄｏｔ－ＥＬＩＳＡ检测６５份弓形虫感染者血清、１７６份其他寄生虫感染者血清和７６份对照血清。结果显示，用Ｄｏｔ－ＩＧＳＳ及Ｄｏｔ－ＥＬＩＳＡ检测弓形虫感染者抗体阳性率分别为９８．４６％及９２．３１％，平均抗体滴度分别为１∶３８４及１∶２１８（１∶２０－１∶２５６０）。用Ｄｏｔ－ＩＧＳＳ检测，有２３份血清抗体滴度高于Ｄｏｔ－ＥＬＩＳＡ，有８份低于Ｄｏｔ－ＥＬＩＳＡ。二种方法所测抗体滴度呈直线正相关关系（ｒ＝０．６０８，Ｐ＜０．０１）。二种方法检测其他寄生虫感染者和对照血清，假阳性率分别为５．９５％及１３．８９％。Ｄｏｔ－ＩＧＳＳ操作流程和Ｄｏｔ－ＥＬＩＳＡ相似，但敏感性、特异性均优于Ｄｏｔ－ＥＬＩＳＡ，前者不使用对人体有害的底物，有较好的推广前景。
To compare the sensitivity and specificity of Dot-IGSS and Dot-ELISA for the detection of Toxoplasma gondii antibodies, Tachyzoites Tachyglossus Soluble Protein Antigen was used as a diagnostic antigen, and Dot-IGSS and Dot Sixty-five sera from Toxoplasma gondii, 176 sera from other parasites and 76 sera from controls were tested by ELISA. The results showed that the positive rates of antibodies against Toxoplasma gondii by Dot-IGSS and Dot-ELISA were 98.46% and 92.31% respectively, and the average antibody titers were 1:384 and 1:18 : 2560). With Dot-IGSS, 23 serum antibody titers were higher than Dot-ELISA and 8 were lower than Dot-ELISA. The antibody titers measured by the two methods showed a linear positive correlation (r = 0.608, P <0.01). Two methods to detect other parasites infected and control serum, false positive rates were 5.95% and 13.89%. The Dot-IGSS procedure is similar to that of Dot-ELISA, but its sensitivity and specificity are better than that of Dot-ELISA. The former does not use the substrate harmful to human body and has a good promotion prospect.