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为研究血管内皮生长因子 (VEGF)过度表达在卵巢癌转移中的作用及可能机制 ,以脂质体介导 VEGF c DNA转染 2株卵巢癌细胞系 Ca OV- 3、COC1,经逆转录聚合酶链式反应 (RT- PCR)、Western blot检测转染 VEGF c DNA前后癌细胞 VEGF、明胶酶 A(MMP- 2 ) m RNA及蛋白表达水平 ;Boyden小室体外侵袭实验比较 VEGF c DNA转染 2株癌细胞前后通过人工重组基底膜的能力变化。结果显示 :VEGF c DNA转染后 2株卵巢癌细胞 VEGF、MMP- 2在 m RNA及蛋白表达水平均较转染前明显增强。Boyden小室体外侵袭实验表明转染 VEGF c DNA的 Ca OV- 3和 COC1穿过人工重组基底膜的相对百分率为 (42 .5± 4 .1) %、 (2 6 .8± 2 .4 ) % ,较对照组穿膜细胞相对百分率 (2 4 .7± 1.9) %、 (8.6±1.1) %明显增加。提示微环境中 VEGF参与了卵巢癌浸润、转移 ,其机制可能与诱使 MMP- 2合成及分泌增加有关
To investigate the role and possible mechanism of vascular endothelial growth factor (VEGF) overexpression in ovarian cancer metastasis, two ovarian cancer cell lines, Ca OV-3, COC1, were transfected with liposome-mediated VEGFc DNA by reverse transcription polymerase chain reaction Enzyme-linked immunosorbent assay (RT-PCR) and Western blot were used to detect the expression levels of VEGF, MMP-2 mRNA and protein before and after VEGFc DNA transfection. Boyden chamber invasion assay compared VEGFc DNA transfection 2 The ability of plant cancer cells to undergo the artificial recombination of the basement membrane before and after the change. The results showed that the expression of VEGF and MMP-2 mRNA and protein in two ovarian cancer cells after VEGF c DNA transfection were significantly higher than those before transfection. The in vitro invasion assay of Boyden chamber showed that the relative percentages of Ca OV-3 and COC1 transfected with VEGF cDNA transfected artificial recombinant basement membrane were (42.5 ± 4.1)%, (22.6 ± 2.4)% , Which was significantly higher than that of control group (24.7 ± 1.9)%, (8.6 ± 1.1)%. Prompted that microenvironment VEGF involved in the invasion and metastasis of ovarian cancer, the mechanism may be associated with induced MMP-2 synthesis and secretion increased