论文部分内容阅读
大豆细胞质雄性不育系的获得,为大豆杂种优势利用奠定了基础。在确认RN型大豆细胞质雄性不育系属单基因配子体不育,恢复性是由显性单基因控制的遗传模式的基础上,开展恢复基因的分子标记研究,旨在找到与恢复基因紧密连锁的分子标记,为进一步克隆恢复基因及恢复系的分子标记辅助育种奠定基础。研究选用412对SSR引物,利用RN型不育系YA与恢复系167杂交的F2分离群体,获得了与恢复基因连锁的两个标记Satt414和Satt596,遗传距离分别为16.4和14.6cM。为了找到更近的分子标记,分析了Satt414和Satt596附近的所有SSR引物,并利用两个遗传差异较大的亲本重新构建了分离群体,从而获得了与恢复基因连锁比较紧密的标记Satt547,遗传距离为7.56cM。根据Cregan等构建的大豆分子遗传连锁图,将恢复基因定位于J连锁群上。
The availability of soybean cytoplasmic male sterile lines laid the foundation for the utilization of soybean heterosis. It is confirmed that RN genotype cytoplasmic male sterility (CMS) genic male sterility is a single gene gametophyte sterility, restoring is based on the dominant single gene controlled genetic model based on the study of molecular marker of restorer gene, aimed at finding a close linkage with the restorer gene Molecular markers, and lay the foundation for molecular marker-assisted breeding for further cloning recovery genes and restorer lines. Two hundred and seventy-two pairs of SSR primers were selected and two markers Satt414 and Satt596 linked to the restorer gene were obtained by F2 segregation population crossed with restorer line 167 and YA sterile line. The genetic distances were 16.4 and 14.6 cM, respectively. In order to find more recent molecular markers, we analyzed all SSR primers near Satt414 and Satt596 and reconstructed the segregation population with two parents with a large genetic difference to obtain a marker Satt547 which is more closely linked to the restorer gene. The genetic distance Is 7.56cM. According to the molecular genetic linkage map of soybean constructed by Cregan et al., The restorer gene is located on the J linkage group.