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目的观察补体片段C3f对人胚肺成纤维细胞(MRC-5)合成α-平滑肌肌动蛋白(α-SMA)的影响。方法将低中高3个不同质量浓度(15.6,62.5,250 ng·mL-1)人工合成的补体片段C3f(实验组)加入培养基中,以刺激MRC-5细胞,以细胞免疫组化法检测α-SMA在MRC-5细胞质中的表达情况。同时,设立对照组:10 ng·mL~(-1)转化生长因子-β_1(TGF-β_1)和空白组(浓度为0的C3f),进行对比研究。结果中剂量实验组与对照组(TGF-β_1)刺激MRC-5细胞合成α-SMA量分别为7.46±0.66,7.54±0.51,差异无统计学意义(P>0.05),说明2组的量相当,表明C3f具有刺激MRC-5合成α-SMA的功能。低中高3个不同质量浓度的细胞内α-SMA表达量分别为4.95±0.78,7.46±0.66和9.23±0.21,显示C3f的浓度与α-SMA的表达量呈正相关(P<0.05),表明C3f具有促进MRC-5表达α-SMA的功能。结论补体片段C3f能够刺激MRC-5细胞内α-SMA的合成增加。
Objective To investigate the effects of C3f complement on α-smooth muscle actin (α-SMA) in human embryonic lung fibroblasts (MRC-5). Methods C3f (experimental group), which was a synthetic low, medium and high concentration of 15.6, 62.5 and 250 ng · mL-1, was added to the culture medium to stimulate MRC-5 cells and detected by immunohistochemistry α-SMA expression in MRC-5 cytoplasm. At the same time, we set up control group: 10 ng · mL -1 transforming growth factor-β 1 (TGF-β 1) and blank group (C3f concentration 0). Results The amount of α-SMA synthesized by TGF-β 1 in MRC-5 cells was 7.46 ± 0.66 and 7.54 ± 0.51, respectively, with no significant difference (P> 0.05), indicating that the amount of α-SMA in MRC- , Indicating that C3f has the function of stimulating the synthesis of α-SMA by MRC-5. The expression of α-SMA in low, middle and high concentrations of three different concentrations were 4.95 ± 0.78, 7.46 ± 0.66 and 9.23 ± 0.21, respectively. The results showed that there was a positive correlation between the concentration of C3f and the expression of α-SMA (P <0.05) Has the function of promoting α-SMA expression of MRC-5. Conclusion Complement C3f can stimulate the synthesis of α-SMA in MRC-5 cells.