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应用光谱法研究在模拟动物生理条件(pH=7.40的缓冲溶液)下,维生素C(Vitamin c)和拉米夫定(Lamivudine)对牛血清白蛋白(BSA)的荧光猝灭作用。建立了利用荧光光谱法研究维生素C和拉米夫定间相互作用的方法。用Stern-Volmer方程处理实验数据,发现BSA与药物反应生成新的产物,以静态猝灭为主要猝灭机理,药物与蛋白结合位点数约为1,药物间存在相互作用,增大了药物与蛋白间的结合常数、稳定性,减少了游离型药物含量,造成药效减弱。
The fluorescence quenching of bovine serum albumin (BSA) by Vitamin C and Lamivudine was studied under the physiological conditions of animals (pH = 7.40 buffer solution). A method to study the interaction between vitamin C and lamivudine by fluorescence spectroscopy was established. Stern-Volmer equations were used to deal with the experimental data. It was found that BSA reacts with the drug to generate new products. Static quenching is the main quenching mechanism. The number of drug-protein binding sites is about 1, interaction between drugs increases, Protein binding constants, stability, reducing the free-type drug content, resulting in reduced efficacy.