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目的 寻找胶质母细胞瘤 (GBM) 4号染色体上可能存在肿瘤抑制基因的杂合性丢失 (LOH)区域 ,为发现和定位肿瘤抑制基因 (TSG)提供线索和依据。方法 应用聚合酶链反应 (PCR)方法 ,采用荧光标记引物和 337型DNA序列自动分析仪 ,分析了2 0例GBM 4号染色体上 2 2个微卫星多态性标记的LOH。结果 在 2 0例GBM中 ,8例存在 4号染色体的LOH ,在 2 1 8% ( 6 1/2 80 )可提供信息位点存在LOH。其中 4q和 4p的LOH分别出现 8例和 5例。GBM中在 4q上的下列位点检测到较高的LOH率 :4q35上的D4s4 2 6 ( 55 6 % ) ,4q31 1- 33上的D4s4 13( 41 6 % )～D4s1597( 40 0 % ) ,4q2 4 - 2 8上的D4s4 0 2 ( 38 5% )～D4s1575( 33 3% )。结论 4号染色体可能在GBM的分子发病机制中发挥着重要作用 ,在 4q35上的D4s4 2 6位点、4q31 1- 33上的D4s4 13～D4s1597、4q2 4 -2 8上的D4s4 0 2～D4s1575间区域可能存在多个与GBM相关的肿瘤抑制基因
OBJECTIVE: To find out the loss of heterozygosity (LOH) region of tumor suppressor gene on chromosome 4 of glioblastoma (GBM), and to provide a clue and basis for the discovery and localization of tumor suppressor gene (TSG). Methods Two hundred and twenty microsatellite loci (LOH) markers on 20 GBM chromosome 4 were analyzed by polymerase chain reaction (PCR) method using fluorescent labeled primers and 337 DNA sequence autoanalyzer. Results In 20 GBMs, there were 8 cases of LOH on chromosome 4, and LOH was found in 21 8% (6 1/2 80) of the available information sites. There were 8 cases and 5 cases in LOH of 4q and 4p respectively. Higher LOH rates were detected in the GBM at 4q4 at D4s4 2 6 (55 6%) on 4q35, D4s 13 13 (41 6%) to D4s 1597 (40 0%) on 4q31 1-33, D4s4 0 2 (38 5%) ~ D4s 1575 (33 3%) on 4q2 4 - 2 8. Conclusion Chromosome 4 may play an important role in the molecular pathogenesis of GBM. D4s4 26 site on 4q35, D4s4 13 ~ D4s1597 on 4q31 1-33, D4s4 0 2 ~ D4s1575 on 4q2 4 -28 There may be multiple GBM-related tumor suppressor genes in the intergenic region