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选择Biolog技术方法,利用方差分析和主成分分析研究了不同矿化度水滴灌下塔里木沙漠公路防护林地土壤微生物的代谢活性。结果表明:随着培养时间的推移,微生物利用单一碳源的颜色平均变化率(AWCD)呈现升高趋势。培养48h内小于0.5,处于缓慢增长期;48h后进入对数增长期;216h后进入稳定期;衰亡期不明显。随着滴灌水矿化度增大,AWCD值明显降低,这种差异主要由土壤微生物对胺类、酚类化合物、碳水化合物利用的差异所致。土壤微生物群落利用碳源活性的垂直差异明显,70~100cm土层是AWCD值最高的区域,表层0~5cm最小。AWCD值与土壤有机质、全氮、全磷、全钾、速效钾、速效磷的含量、pH值、阳离子交换量、容重的正相关系数均大于0.85。因此,土壤微生物利用碳源的代谢活性差异主要由土壤化学性质所致,土壤全量养分和酸碱度是主要驱动因子。胺类、酚类化合物、碳水化合物是区分不同矿化度水滴灌下土壤微生物群落生态功能的敏感碳源。
Biolog method was used to analyze the metabolic activity of soil microorganisms in Tarim Desert Highway shelterbelts with different salinities of drip irrigation by using ANOVA and principal component analysis. The results showed that with the increase of incubation time, the average color change rate (AWCD) of microbial single carbon source showed an increasing trend. Less than 0.5 within 48h after culture, in slow growth phase; 48h after entering the logarithmic growth phase; 216h after entering the stable phase; decay period was not obvious. With the increase of drip irrigation salinity, the AWCD value decreased obviously. The difference was mainly caused by the differences of soil microorganisms on the utilization of amines, phenols and carbohydrates. The vertical difference of carbon source utilization in soil microbial community was obvious. The area with the highest AWCD value in the 70 ~ 100 cm soil layer was the lowest in 0 ~ 5 cm surface layer. AWCD value and soil organic matter, total nitrogen, total phosphorus, total potassium, available potassium, available phosphorus content, pH, cation exchange capacity, bulk density positive correlation coefficient greater than 0.85. Therefore, the difference in metabolic activity of soil microbial utilization of carbon sources is mainly caused by the soil chemical properties, and the total nutrient and pH of soil are the main driving factors. Amines, phenolic compounds and carbohydrates are sensitive carbon sources for distinguishing the ecological functions of soil microbial communities under different salinities.