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目的 在尿激酶溶解大鼠脑血栓治疗中，研究亚低温对溶栓复流后大脑中动脉缺血区 Ｐ５３蛋白表达的影响。方法 应用肾血管性高血压大鼠（ Ｒ Ｈ Ｒ Ｓ Ｐ），用光化学法制成一侧大脑中动脉闭塞（ Ｍ Ｃ Ａ Ｏ）模型，在血栓形成后 ０．５ｈ 应用尿激酶静脉溶栓复流后，用免疫组织化学的方法研究 Ｐ５３蛋白的表达。结果 亚低温组 Ｐ５３蛋白的表达，明显弱于正常体温组。结论 亚低温降低脑缺血区域的 Ｐ５３蛋白的表达，可能是亚低温产生脑保护的机制之一。
Objective To investigate the effect of mild hypothermia on the expression of P53 protein in ischemic area of middle cerebral artery after thrombolytic therapy in urokinase-treated rats. Methods Renal vascular hypertensive rats (R H R S P) were used to make a model of middle cerebral artery occlusion (M C A O) by photochemical method. After 0.5 h of thrombus formation, , The expression of P53 protein was studied by immunohistochemistry. Results The expression of P53 protein in the hypothermia group was significantly weaker than that in the normal body temperature group. Conclusion Mild hypothermia can reduce the expression of P53 protein in cerebral ischemic area, which may be one of the mechanisms of brain protection induced by mild hypothermia.