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【目的】了解新疆彩色丘陵地貌中不同颜色岩石层的细菌多样性及其优势种群。【方法】利用末端限制性片段长度多态性(Terminal Restriction Fragment Length Polymorphism,T-RFLP),对5种不同颜色岩石层细菌多样性进行分析。【结果】T-RFLP图谱和聚类分析结果显示,2号(粉红色)岩石层细菌多样性最高,2号(粉红色)和3号(红灰色)样品中细菌群落结构相似性最高,而5号(橙棕色)样品与其他样品间相似性最低,各样品间细菌群落结构相似性介于5.6%~60%;所得结果与RDP数据库比对,推测出5种岩石样品的优势微生物种群分别为Ruminococcaceae,Burkholderiaceae,iron-reducing bacterium,Shewanella,Frateuria和一些免陪养菌群。【结论】新疆彩色丘陵地貌岩石层样品随着颜色由浅至深,其末端限制性片段(Terminal RestrictionFragments,TRFs)的数量由多到少,除5号(橙棕色)外,其余岩石层细菌多样性较高;由分析结果可以推测,新疆彩色丘陵地貌岩石层中可能存在一些潜在新种。
【Objective】 The objective of this study was to understand the bacterial diversity and its dominant populations in different color rock layers in the colorful hilly landscape of Xinjiang. 【Method】 Bacterial diversity in five different color rock layers was analyzed by using Terminal Restriction Fragment Length Polymorphism (T-RFLP). 【Result】 The results of T-RFLP and cluster analysis showed that the bacterial diversity was the highest in No.2 (pink) lithosphere, and the highest similarity was found in No.2 (pink) and No.3 (red-gray) The similarity between the 5 samples (orange-brown) and other samples was the lowest, and the bacterial community structure similarity among samples was 5.6% -60%. The obtained results were compared with the RDP database to infer that the dominant microbial populations of 5 samples Ruminococcaceae, Burkholderiaceae, iron-reducing bacterium, Shewanella, Frateuria, and some parasite-free flora. 【Conclusion】 The results showed that the color of rock samples from colored hilly landform in Xinjiang was from light to deep, and the number of Terminal Restriction Fragments (TRFs) decreased from more to less. The bacterial diversity of other rock layers except for No. 5 (orange-brown) It can be inferred from the analysis results that there are some potential new species in the rock layers of colored hilly landform in Xinjiang.