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目的研究麦芽醇对大鼠海马组织不完全性脑缺血再灌注损伤的保护作用。方法Wistar大鼠双侧颈总动脉夹闭 30min ,造成不完全脑缺血 ,松开动脉夹即为再灌注。大鼠随机分为三组 :①假手术对照组 (SOC) ,相同手术操作 ,但不夹闭动脉 ;②缺血再灌注组 (IR) ,再灌注 1h ;③麦芽醇治疗组 (MT) ,夹闭动脉前后静脉注射 70 0 μmol/L麦芽醇生理盐水。 3组动物均于再灌注后测海马组织中的丙二醛(MDA)值、超氧化物歧化酶 (SOD)和ATP酶活性。结果缺血再灌注组MDA明显升高 (P <0 .0 1 ) ,SOD、ATP酶活性明显降低 (P <0 .0 1 )。MT能降低升高的MDA值 ,改善ATP酶活性 (P <0 .0 1 ) ,但不能改善SOD活性。结论麦芽酶能清除氧自由基 ,减轻脂质过氧化反应 ,改善ATP酶活性 ,从而减轻海马组织缺血再灌注损伤。
Objective To study the protective effect of maltol on incomplete cerebral ischemia-reperfusion injury in rat hippocampus. Methods Wistar rat bilateral common carotid artery was occluded for 30 minutes, which resulted in incomplete cerebral ischemia. The release of the arterial clamp was reperfusion. Rats were randomly divided into three groups: 1 sham-operated control group (SOC), same surgical procedure but no arterial clamping; 2 ischemia-reperfusion group (IR), reperfusion 1 h; 3 maltol treatment group (MT), Before and after arterial clamping, 70 μmol/L maltol normal saline was intravenously injected. The malondialdehyde (MDA) value, superoxide dismutase (SOD) and ATPase activity in the hippocampus of the three groups of animals were measured after reperfusion. RESULTS: In the ischemia-reperfusion group, MDA was significantly increased (P < 0.01), and SOD and ATPase activities were significantly decreased (P < 0.01). MT reduced the elevated MDA value and improved ATPase activity (P < 0.01) but did not improve SOD activity. Conclusion Maltase can scavenge oxygen free radicals, reduce lipid peroxidation, improve ATPase activity, and reduce ischemia-reperfusion injury in hippocampus.