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目的:探讨 Ag N O R 定量诊断和免疫细胞化学技术对浆膜腔转移性腺癌细胞的诊断价值。方法:应用 Ag N O R定量诊断和 C E A、 C K 及 V I M 免疫细胞化学技术对 56 例浆膜腔积液进行对比研究。结果:转移性腺癌细胞与间皮细胞之间的 Ag N O R 计数,颗粒形态及 C E A 和 V I M 的表达均有显著性差异,而且 Ag N O R 定量诊断与 C E A 表达呈线性正相关,与 V I M 表达呈线性负相关。结论: Ag N O R定量研究与 C E A 和 V I M 免疫细胞化学染色联合应用对浆膜腔转移性腺癌细胞的诊断及鉴别诊断有重要价值。
Objective: To investigate the diagnostic value of Ag N O R quantitative diagnosis and immunocytochemistry for serous cavity metastatic adenocarcinoma cells. Methods: Quantitative diagnosis of Ag N O R and C E A, C K and V I M immunocytochemistry were used to compare 56 serous effusions. Results: There were significant differences in Ag N O R counts, granular morphology and expression of C E A and V I M between metastatic adenocarcinoma cells and mesothelial cells. Quantitative diagnosis of Ag N O R and expression of C E A were shown. Linear positive correlation and linear negative correlation with V I M expression. Conclusion: The quantitative study of Ag N O R combined with the immunocytochemical staining of C E A and V I M has important value in the diagnosis and differential diagnosis of serous cavity metastatic adenocarcinoma cells.