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通过将羧化聚苯乙烯乳胶与大肠杆菌O157:H7 Intimin蛋白单克隆抗体进行偶联,对最佳偶联抗体IgG量、最佳偶联时间等条件进行优化,建立了快速检测大肠杆菌O157:H7的乳胶凝集试验方法。结果表明,该方法具有快速、敏感、特异、简便等优点,适于临床应用,为大肠杆菌O157:H7的快速诊断提供了技术支撑。
By coupling the carboxylated polystyrene latex with the monoclonal antibody of E. coli O157: H7 Intimin, we optimized the conditions such as the optimal amount of conjugated antibody (IgG), the optimum coupling time and so on. The rapid detection of E. coli O157: H7 latex agglutination test method. The results showed that the method was rapid, sensitive, specific and simple, which was suitable for clinical application and provided technical support for the rapid diagnosis of Escherichia coli O157: H7.