目的 :制备并纯化抗核糖核酸酶抑制因子 (RI)的抗体。方法 :从人胎盘中提取RI,经亲和层析纯化后免疫家兔制备RI抗体。用rProteinASepharoseFastFlow层析对抗RI抗体进行纯化 ,并用SDS PAGE、ELISA及Westernblot等进行鉴定。结果 :制备并纯化了抗RI抗体 ,经SDS PAGE分析达到了电泳纯。结论 :获得了效价高、特异性强、稳定性好的抗RI抗体 ,为对其深入研究奠定了基础 OBJECTIVE: To prepare and purify anti-ribonuclease inhibitor (RI) antibodies. Methods: RI was extracted from human placenta and purified by affinity chromatography to prepare RI antibody. The RI antibodies were purified by rProtein Sepharose Fast Flow Chromatography and identified by SDS PAGE, ELISA and Western blotting. Results: The anti-RI antibody was prepared and purified and electrophoresed pure by SDS PAGE. Conclusion: The anti-RI antibody with high titer, specificity and stability is obtained, which lays the foundation for further research