论文部分内容阅读
以豌豆幼苗为试材,采用半定量和实时荧光定量RT-PCR技术,研究了不同浓度PEG 6000对肌动蛋白异型体PEAc3表达的影响,为深入了解豌豆幼苗抗旱分子机理以及筛选耐旱豌豆品种奠定基础。结果表明:与正常供水处理相比,15%PEG 6000处理下PEAc3基因的表达量略有升高,20%PEG 6000处理下PEAc3基因的表达量显著升高,低于10%PEG 6000处理,PEAc3的表达量基本没有变化,而25%PEG 6000处理下PEAc3基因的表达量则有所下降。不同干旱条件下PEAc3基因表达量不同这一结果表明,PEAc3基因可能参与豌豆幼苗适应干旱胁迫的生理生化过程,该基因有可能用于豌豆抗旱性强弱的分子筛选。
Using pea seedlings as test material, the effect of different concentrations of PEG 6000 on the expression of actin PEAc3 was studied by semi-quantitative and real-time fluorescent quantitative RT-PCR. To understand the molecular mechanism of drought resistance in pea seedlings and to screen drought-tolerant pea varieties Lay the foundation. The results showed that the expression level of PEAc3 gene increased slightly under 15% PEG 6000 treatment compared with that of normal water supply. The expression level of PEAc3 gene under 20% PEG 6000 treatment was significantly lower than that of PEAc3 treated with PEG 6000 The expression level of PEAc3 gene decreased with 25% PEG 6000 treatment. The results of different expression levels of PEAc3 under different drought conditions indicate that PEAc3 may be involved in physiological and biochemical processes of pea seedlings adapted to drought stress and may be used in molecular screening of pea drought resistance.