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应用30对COSII引物对刚竹属4个竹种及其一个栽培变型进行PCR扩增,电泳结果显示,所有引物都可以在至少3个竹种中获得特异性PCR产物,共扩增出101条带,其中88条表现出多态性,占87.13%。其中2对引物在5份材料中均扩增出一致的多拷贝条带。利用NTsys 2.10e软件,对5个供试竹种材料的亲缘关系进行了分析,从聚类结果看,当遗传系数为0.658时,5个供试材料可以分为3类,紫竹与花哺鸡竹各自单独成一类,桂竹、罗汉竹和斑竹的亲缘关系比较近,形成第3类。这一分类结果与形态学分类结果大致吻合,表明基于水稻等禾本科植物的基因组序列开发出来的COSII分子标记在竹类植物中具一定通用性,且可用于鉴定竹种间的亲缘关系。
30 pairs of COSII primers were used for PCR amplification of 4 bamboo species and one cultivated variant of the genus Phyllostachys. The electrophoresis results showed that all the primers could obtain specific PCR products in at least 3 species of bamboo, and a total of 101 Among them, 88 showed polymorphism, accounting for 87.13%. Two pairs of primers amplified the same multi-copy bands in five materials. Using NTsys 2.10e software, we analyzed the genetic relationship of five tested bamboo materials. According to the clustering results, when the genetic coefficient was 0.658, the five tested materials could be divided into three categories, Bamboo into a separate category, Guizhu, Rohan Bamboo and Bamboo relatives closer to form the third category. The results of this classification are in good agreement with the results of morphological classification, indicating that the COSII markers developed based on the genomes of gramineous rice and other grasses have some commonality in bamboo plants and can be used to identify the genetic relationship between bamboo species.